作者
Jong-Eun Park,Rachel A. Botting,Cecilia Domínguez Conde,Dorin-Mirel Popescu,Marieke Lavaert,Daniel J Kunz,Issac Goh,Emily Stephenson,Roberta Ragazzini,Elizabeth Tuck,Anna Wilbrey-Clark,Kenny Roberts,Veronika R. Kedlian,John R. Ferdinand,Xiaoling He,Simone Webb,Daniel Maunder,Niels Vandamme,Krishnaa T. Mahbubani,Krzysztof Polanski,Lira Mamanova,Liam Bolt,David Crossland,Fabrizio De Rita,Andrew Fuller,Andrew Filby,Gary Reynolds,David Dixon,Kourosh Saeb-Parsy,Steven Lisgo,Deborah J. Henderson,Roser Vento-Tormo,Omer Ali Bayraktar,Roger A. Barker,Kerstin B. Meyer,Yvan Saeys,Paola Bonfanti,Sam Behjati,Menna R. Clatworthy,Tom Taghon,Muzlifah Haniffa,Sarah A. Teichmann
摘要
The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We used single-cell RNA sequencing to create a cell census of the human thymus across the life span and to reconstruct T cell differentiation trajectories and T cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ CD8αα+ T cell populations, thymic fibroblast subtypes, and activated dendritic cell states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and the kinetics of lineage commitment. Taken together, our data provide a comprehensive atlas of the human thymus across the life span with new insights into human T cell development.