Immunohistochemistry and Detection of Proliferating Cells by BrdU

Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in ­tissue. The method exploits the principle that the target antigen is recognized by specific antibody and is visualized using different detection systems. The subject of this chapter is simultaneous immunohistochemical detection of protein antigens and proliferation marker BrdU in the developing tooth.