三氯乙酸
骨脱钙
丙酮
萃取(化学)
蛋白质沉淀
化学
降水
色谱法
盐酸
蛋白质纯化
生物化学
无机化学
牙科
医学
物理
气象学
作者
Yongfeng Cui,Yang Zhen,Bo Zhu,Xiao Lu-wei,Peijian Tong
出处
期刊:PubMed
日期:2011-04-01
卷期号:33 (2): 210-3
被引量:1
标识
DOI:10.3881/j.issn.1000-503x.2011.02.022
摘要
To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA)-acetone precipitation method.Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction. The efficiencies of these two methods were compared.TCA-acetone precipitation method had significantly higher extraction efficiency. Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue. Protein band distribution was similar between these two methods.TCA-acetone precipitation method is useful for bone proteomics research.
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