Therapeutic effects of resveratrol on memory deficits in offspring of sleep-deprived rats: Involvement of hippocampal BDNF-TrkB pathways

Background: Maternal sleep deprivation (MSD) is a significant public health issue that adversely affects neurogenesis and synaptic plasticity in offspring, resulting in cognitive deficits in learning and memory. Resveratrol, an antioxidant with neuroprotective and anti-inflammatory properties, may help mitigate these effects. This study investigates resveratrol’s potential to counteract the negative impacts of MSD on neurodevelopment in male Wistar rat offspring. Methods: Ninety-six male Wistar rat offspring and 36 pregnant rats were used. Total MSD was induced using the water box device on gestational days 7, 11, and 17. Pregnant rats received resveratrol at doses of 25 or 50 mg/kg every 12 h during the sleep deprivation period. After parturition, offspring were divided into 12 groups for assessment at two months of age. Social interaction tests evaluated social memory, while the Morris water maze test assessed spatial learning and memory. Brain samples were prepared for Nissl staining, and brain-derived neurotrophic factor (BDNF) and tyrosine-protein kinase (TrkB) expression levels in the hippocampus were measured using western blotting. Results: Our findings indicate that the MSD group exhibited decreased BDNF/TrkB expression and increased neuronal damage in the hippocampus, which led to disrupted spatial and social memory compared to the control group. Subsequently, resveratrol administration, especially at a dose of 50 mg/kg during pregnancy, significantly reversed MSD’s detrimental effects on cognitive function in offspring. Conclusion: Our results provide novel evidence of resveratrol’s neuroprotective effects in rat pregnancy models of MSD, suggesting its potential for developing therapeutic interventions targeting prenatal neurodegenerative disorders.