生物
细胞生物学
单核细胞
祖细胞
免疫学
细胞分化
巨噬细胞
骨髓
单核吞噬细胞系统
干细胞
体外
基因
遗传学
作者
Domien Vanneste,Boqin Qiang,Shakir Hasan,Peng Wen,Dimitri Pirottin,Joey Schyns,Philippe Maréchal,Cecilia Ruscitti,Margot Meunier,Zhaoyuan Liu,Céline Legrand,Laurence Fiévez,Florent Ginhoux,Coraline Radermecker,Fabrice Bureau,Thomas Marichal
标识
DOI:10.1038/s41590-023-01468-3
摘要
Abstract Resident tissue macrophages (RTMs) are differentiated immune cells that populate distinct niches and exert important tissue-supportive functions. RTM maintenance is thought to rely either on differentiation from monocytes or on RTM self-renewal. Here, we used a mouse model of inducible lung interstitial macrophage (IM) niche depletion and refilling to investigate the development of IMs in vivo. Using time-course single-cell RNA-sequencing analyses, bone marrow chimeras and gene targeting, we found that engrafted Ly6C + classical monocytes proliferated locally in a Csf1 receptor-dependent manner before differentiating into IMs. The transition from monocyte proliferation toward IM subset specification was controlled by the transcription factor MafB, while c-Maf specifically regulated the identity of the CD206 + IM subset. Our data provide evidence that, in the mononuclear phagocyte system, the ability to proliferate is not merely restricted to myeloid progenitor cells and mature RTMs but is also a tightly regulated capability of monocytes developing into RTMs in vivo.
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