Less is more: A new strategy combining nanomaterials and PGPB to promote plant growth and phytoremediation in contaminated soil

Novel combination strategies of nanomaterials (NMs) and plant growth-promoting bacteria (PGPB) may facilitate soil remediation and plant growth. However, the efficiency of the NM-PGPB combination and interactions among NMs, PGPB, and plants are still largely unknown. We used multiwalled carbon nanotubes (MWCNTs) and zero-valent iron (nZVI) combined with Bacillus sp. PGP5 to enhance the phytoremediation efficiency of Solanum nigrum on heavy metal (HM)-contaminated soil. The NM-PGPB combination showed the best promoting effect on plant growth, which also had synergistic effects on the bioaccumulation of HMs in S. nigrum. The MWCNT-PGP5 combination increased the Cd, Pb, and Zn removal efficiency of S. nigrum by 62.03%, 69.44%, and 61.31%, respectively. The underlining causes of improved plant growth and phytoremediation by NMs-PGPB combination were further elucidated. NM application promoted PGPB survival in soil. Compared with each single application, the combined application minimized disturbance to plant transcription levels and rhizosphere microbial community, resulting in the best performance on soil remediation and plant growth. The NM-PGPB-induced changes in the microbial community and root gene expression were necessary for plant growth promotion. This work reveals the "less is more" advantage of the NM-PGPB combination in soil remediation, providing a new strategy for soil management. Heavy metal contamination is a global environmental issue. Here, we propose a novel strategy that combines nanomaterials (NMs) with PGPB to promote safe bioremediation of contaminated soils. NM application promoted PGPB survival in contaminated soil. The NM-PGPB combination performed best in promoting the growth and phytoremediation of Cd-hyperaccumulator Solanum nigrum, while minimized disturbance to plant transcription levels and rhizosphere microbial community. The work provides a new solution for soil management that "less is more" for soil remediation.