While AI has been used in health sciences libraries for decades, the emergence of publicly available large language models (LLMs) has the potential to change how researchers conduct literature searches for systematic reviews. Using a recently published systematic review as a model, we compared the review's published Medline OVID search strategy with 3 strategies that ChatGPT created when prompted with the review's objective. Then we ran the published review and each LLM search strategy through Medline to compare the results with the articles identified by the review's authors to be important.