医学
外渗
血红蛋白
梗塞
麻醉
生理盐水
大脑中动脉
脑梗塞
脂质体
血液替代品
血栓形成
大脑皮层
内科学
病理
缺血
生物化学
化学
心肌梗塞
作者
Akira Kawaguchi,Dai Fukumoto,Munetaka Haida,Yoshitaka Ogata,Mariko Yamano,Hideo Tsukada
出处
期刊:Stroke
[Ovid Technologies (Wolters Kluwer)]
日期:2007-05-01
卷期号:38 (5): 1626-1632
被引量:76
标识
DOI:10.1161/strokeaha.106.467290
摘要
Liposome-encapsulated hemoglobin (LEH; TRM-645) is a novel oxygen (O(2)) carrier with a lower O(2) affinity (P(50)O(2)=40 mm Hg) than red blood cells. In contrast to cell-free hemoglobin, encapsulation prevents hemoglobin extravasation, whereas its subcellular size (230 nm) may improve O(2) delivery and limit the severity of cerebral infarction.The extent of cerebral infarction was determined 24 hours after photochemically induced thrombosis of the middle cerebral artery from the integrated area of infarction detected by triphenyltetrazolium chloride staining in rats receiving no treatment, 10 mL/kg of LEH, homologous blood, empty liposomes, or saline. To develop a dose-response relationship, LEH dose was reduced from 10 mL/kg to 2 mL/kg, 0.4 mL/kg, and 0.08 mL/kg.Infarction extent was significantly suppressed in rats receiving LEH as compared with animals receiving no infusion, saline, empty liposome, or transfusion in the cortex but not in the basal ganglia, where all had similar degrees of damage. The dose-response relationship revealed that as little as 2 mL/kg of LEH was protective, whereas the total blood O(2) content, hemoglobin level, and transfusion and/or infusion of empty liposomes or saline were not effective.Our results suggest that LEH significantly reduces the area of infarction in the cortex but not in basal ganglia after photochemically induced thrombosis of the middle cerebral artery in the rat.
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