Response to Comment on "PDK1 Nucleates T Cell Receptor-Induced Signaling Complex for NF-κB Activation"

In their comment, Gruber et al . report constitutive phosphorylation of protein kinase C θ at threonine residue 538. However, they fail to note that, consistent with our results, a number of other groups have previously reported inducible phosphorylation of Thr-538 in T cells. Although the physiological relevance of this discrepancy is unclear, substantial differences in experimental conditions and reagents may account for the conflicting observations.