化学
氧化应激
程序性细胞死亡
谷胱甘肽
抗氧化剂
活力测定
过氧化氢酶
脂质过氧化
细胞生物学
细胞毒性
植物
细胞内
超氧化物歧化酶
园艺
毒性
丙二醛
作者
Aili Wei,Baocun Fu,Yunshan Wang,Xiaoyan Zhai,Xiaojing Xin,Chao Zhang,Dongmei Cao,Xiaobing Zhang
标识
DOI:10.1016/j.ecoenv.2015.01.024
摘要
Both nitric oxide (NO) and reactive oxygen species (ROS) are very important signal molecules, but the roles they play in signal transduction of sulfur dioxide (SO2) induced toxicities on ornamental plants is not clear. In this study, the functions of NO and ROS in SO2-induced death of lower epidermal guard cells in ornamental plant Tagetes erecta were investigated. The results showed that SO2 derivatives (0.4-4.0 mmol L(-1) of final concentrations) could reduce the guard cells' viability and increase their death rates in a dose-dependent manner. Meanwhile, the significant increase of cellular NO, ROS, and Ca(2+) levels (P<0.05) and typical apoptosis features including nucleus condensation, nucleus break and nucleus fragmentation were observed. However, exposure to 2.0 mmol L(-1) of SO2 derivatives combined with either NO antagonists (NO scavenger c-PTIO; nitrate reductase inhibitor NaN3; NO synthase inhibitor L-NAME), ROS scavenger (AsA or CAT) or Ca(2+) antagonists (Ca(2+) scavenger EGTA or plasma membrane Ca(2+) channel blocker LaCl3) can effectively block SO2-induced guard cells death and corresponding increase of NO, ROS and Ca(2+) levels. In addition, addition of L-NAME or AsA in 2.0 mmol L(-1) of SO2 derivatives led to significant decrease in the levels of NO, ROS and Ca(2+), whereas addition of LaCl3 in them just resulted in the decrease of Ca(2+) levels, hardly making effects on NO and ROS levels. It was concluded that NO and ROS were involved in the apoptosis induced by SO2 in T. erecta, which regulated the cell apoptosis at the upstream of Ca(2+).
科研通智能强力驱动
Strongly Powered by AbleSci AI