DNA折纸
光热治疗
纳米技术
费斯特共振能量转移
纳米颗粒
DNA纳米技术
材料科学
纳米结构
光热效应
荧光
DNA
化学
作者
Chao Zhang,Xinxin Jing,Linjie Guo,Chengjun Cui,Xiaoling Hou,Tingting Zuo,Jiangbo Liu,Jiye Shi,Xiaoguo Liu,Xiaolei Zuo,Jiang Li,Chao Chang,Chunhai Fan,Lihua Wang
出处
期刊:Nano Letters
[American Chemical Society]
日期:2021-06-16
卷期号:21 (13): 5834-5841
被引量:3
标识
DOI:10.1021/acs.nanolett.1c01821
摘要
In situ synthesis of DNA origami structures in living systems is highly desirable due to its potential in biological applications, which nevertheless is hampered by the requirement of thermal activation procedures. Here, we report a photothermal DNA origami assembly method in near-physiological environments. We find that the use of copper sulfide nanoparticles (CuS NPs) can mediate efficient near-infrared (NIR) photothermal conversion to remotely control the solution temperature. Under a 4 min NIR illumination and subsequent natural cooling, rapid and high-yield (>80%) assembly of various types of DNA origami nanostructures is achieved as revealed by atomic force microscopy and single-molecule fluorescence resonance energy transfer analysis. We further demonstrate the in situ assembly of DNA origami with high location precision in cell lysates and in cell culture environments.
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