肉眼
化学
核酸内切酶
多重位移放大
线性范围
小RNA
检出限
脱氧核酶
多路复用
熵(时间箭头)
环介导等温扩增
分子信标
DNA
计算生物学
寡核苷酸
核酸
计算机科学
分子生物学
信号(编程语言)
核糖核酸
滚动圆复制
生物系统
基因
聚合酶链反应
色谱法
生物化学
生物信息学
生物
聚合酶
物理
DNA提取
量子力学
作者
Sha Zhu,Yiqi Yang,Yuedi Ding,Ninghan Feng,Menglu Li,Yongmei Yin
出处
期刊:Talanta
[Elsevier]
日期:2021-12-01
卷期号:235: 122810-122810
被引量:8
标识
DOI:10.1016/j.talanta.2021.122810
摘要
MicroRNAs (miRNAs) are currently recognized as novel biomarkers for cancer early diagnosis, therapy selection, and progression monitoring. Herein, we developed an ultrasensitive and label-free homogeneous colorimetric strategy for miRNA detection based on engineering entropy-driven amplification (EDA) coupled with nicking enzyme-assisted AuNP aggregation. In our design, the target miRNA could specifically trigger the EDA recycling process. One of the EDA products could open the hairpin probe and form a dual strand containing a nicking endonuclease (Nb.BbvCl) cleavage region. After adding nicking endonuclease in the sensing solution, the product DNA fragments could act as two linkers, inducing the aggregation of ssDNA-modified AuNPs. Simultaneously, the liberating complementary strands continued to cyclic hybridization with the hairpin probe. This multiple signal amplification colorimetric strategy showed a wide linear range from 10 fM to 100 pM with a much lower detection limit of 3.13 fM for miRNA let-7a, which also performed well in a complex sample matrix. Most importantly, the naked eye could clearly distinguish the 10 fM color change caused by let-7a to be measured. Moreover, this approach could easily extend to multiple miRNAs with target-specific sequence substitutions. Therefore, this ultrasensitive visual strategy for miRNA demonstrated attractive potentials for promising applications in clinical diagnosis.
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