纳米器件
脱氧核酶
小RNA
纳米技术
体内
转染
癌症生物标志物
纳米医学
荧光
纳米颗粒
材料科学
化学
基因
DNA
癌症
医学
生物
生物化学
物理
生物技术
量子力学
内科学
作者
Yanmei Zhou,Wenxiao Ma,Ruijiao Sun,Bo Liu,Xiaoru Zhang,Hongsheng Yang
标识
DOI:10.1016/j.bios.2022.114549
摘要
Despite the great promise of cancer theranostic platforms, accurate diagnosis and effective treatment are still highly challenging. In this work, nanodevice for intracellular miRNAs detection and artificially controlled drug releasement was developed based on upconverting nanoparticles (UCNPs). For analysis aspect, DNAzymes amplified miRNA-21 detection was carried out, giving excellent sensitivity with detection limits of 1.8 × 10-11 M. Moreover, intracellular fluorescence imaging permitted in situ diagnoses of miRNA-21 expression in living cells. Once the test identifies tumor markers, treatment can be performed. Here, artificially controlled chemo-gene synergetic therapy nanodevice was obtained by integrating UCNPs with photocleavable linkers (PC-linkers). In vitro and in vivo experiments verified the potential application of prepared nanodevice in cancer theranostics.
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