Bimodal Turn‐On Fluorescent Probe for Photophysical and Electrochemical Detection of Human Serum Albumin in Clinical Samples

Abstract A simple, easy to synthesize, cost‐effective fluorescent turn‐on sensor is developed for the selective detection of human serum albumin (HSA) in both biological and environmental samples. The biosensor is unique with qualities of rapidly quantifying HSA levels both at lower and higher concentrations in divergent bio‐fluids with high sensitivity. This probe has also been successfully used for the quantitative determination of serum albumin levels in a large number of patients’ samples having different clinical manifestations. Electrochemical detection of HSA concentrations can also be achieved with this biosensor using a potentiostat. Thus, this probe offers a unique potential of diagnosing HSA levels directly from patient samples using its bimodal (i.e., photophysical and electrochemical) properties, which are hitherto unknown to date.