5'侧翼区域
基因
促卵泡激素受体
激素受体
受体
生物
激素
细胞生物学
遗传学
促卵泡激素
内分泌学
发起人
基因表达
促黄体激素
癌症
乳腺癌
作者
Jörg Gromoll,Berno Dankbar,Thomas Gudermann
标识
DOI:10.1016/0303-7207(94)90102-3
摘要
A genomic clone containing 2.3 kilobases (kb) of the 5' flanking region of the human follicle-stimulating hormone receptor (FSHR) plus the translated region of exon 1 and subsequent sequences of intron A has been isolated and characterized. This portion of the 5' flanking region has neither a TATA nor a CCAAT box and shows features of promoters seen in "housekeeping" genes. Using RNAse protection multiple transcriptional start sites could be identified, the major ones clustered between -114 and -79 bp. Chimeras containing 1486 bp of the 5' flanking region, or deletions thereof, expressed significant chloramphenicol acetyltransferase (CAT) activity when transiently transfected into Chinese hamster ovary (CHO), primary rat Sertoli and human granulosa-lutein cells. Deletion analyses indicated that a proximal promoter can be allocated to the region from -225 to -1 bp.
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