Objective To inspect the blood physiological and biochemiccal of Rhesus macaque and Long-tailed macaque,analyse their basic characters of biological parameters,and set up their routine.Methods Routine methods and instruments were used to determine the blood physiological and biochemical parameters with MEK-5126K(animal CMOS chip)and backman CX5.The hematophysiological indices were determinated in Rhesus macaque and Long-tailed macaque.Results There are significantly difference in AST/ALT between male and female Rhesus macaque,and significantly differece in Eosinophilla、ALP、CK、GGR、CREA between male and female Long-tailed macaque;There are also significantly difference in WBC、MCH、MCHC、Eosinophilla、Monocytosis、ALB、ALB/GLB、AST、AST/ALT、CK、LDH、GGR、CREA between Rhesus macaque and Long-tailed macaque.Conclusion Biological parameters of Rhesus macaque and Long-tailed macaque are established,which could provide basic data for further study.