Determination of coptisine and epiberberine from different species of Coptis by RP-HPLC

OBJECTIVE To determine the concentration of coptisine,epiberberine from C.deltoidea,C emelensis,C teeta,C teetoides,C. linearisepala,C. chinensis and C. chinensis var.brevisepala.METHODS RP-HPLC was used. RESULTS and CONCLUSION All species of Coptis contained coptisine and epiberberine. The content of coptisine was over 1% . All species of Coptis had the five common peaks: coptisine, epiberberine,berberine,palmatine and jatrorrhizine and the ratio between them was about 1.5∶1∶6∶1.5∶0.8.