Genetic diversity of gliadin in wild germplasm of Roegneria kamoji

Acid polyacrylamide gel electrophoresis(A-PAGE) molecular markers were used to detect the genetic diversity among 42 wild accessions of Roegneria kamoji collected from four regions(Sichuan,Chongqing,Gansu and Japan).1) A total of 29 bands of which 89.66 percent were polymorphic were detected in all accessions.The Nei genetic similarity coefficients of the tested accessions ranged from 0.448 to 0.966,with an average Nei of 0.695.These results suggested that there was rich genetic diversity among the tested wild resources of R.kamoji.2) 42 wild accessions could be clustered into three groups.Moreover,the groups from the same origin frequently clustered into one group suggesting a correlation among the tested wild resources,geographical and ecological environment.3) Genetic differentiation between and within five eco-geographical groups of R.kamoji was estimated by Shannon's diversity index,which showed that 83.69 percent genetic variance existed within groups,and 16.31 percent genetic variance was between groups.4) UPGMA cluster analysis based on Nei's unbiased measures of genetic identity was assayed for six eco-geographical groups of R.kamoji,indicating that there was a correlation between genetic differentiation and eco-geographical habits between the five groups.