Application of atomic force microscope in the study of destructive effect of PDT on invasive membrane structure of glioma cells

Objective To explore the destructive effect ofphotodynamic therapy(PDT)on the invasive specialized structures on the surface of glioma cells.Methods Nano-scale morphological contrast study of specialized membrane structures on the surface of U251 glioma cells before and after being acted on by 5-aminolevulinic acid(5-ALA)was performed through the application of cell culture and atomic force microscopy.Results The abundance of pseudopodia and microvillus structures on the surface of glioma cell membrane,which used to be about 400 nm in diameter,1μm in length and finger-like protuberance became spherical granules with the diameter of 300nm,and finger-like protuberance and membraneous pseudopodia and filopodia disappeared,after 5-ALA-induced PDT. Conclusion The 5-ALA-induced PDT has significant destruction effect on invasiveness of glioma cells.