原位
小RNA
寡核苷酸
计算生物学
锁核酸
原位杂交
生物
化学
遗传学
基因表达
基因
有机化学
出处
期刊:Methods in molecular biology
日期:2009-09-06
卷期号:: 127-136
被引量:11
标识
DOI:10.1007/978-1-60327-005-2_9
摘要
A spatial and temporal analysis of miRNA accumulation by in situ analyses is the prerequisite of understanding the precise biological functions of miRNAs. Since miRNAs are very short molecules, their in situ analysis is technically demanding. Here, we describe a protocol for miRNA in situ detection in plants based on LNA-modified oligonucleotides probes. LNA modification significantly enhances the sensitivity and specificity of miRNA detecting probes and provides relatively easy in situ miRNA detection.
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