Optimization of Extraction Method of Vinblastine Catharanthine and Vindoline from Fresh Catharanthus Roseus

Objective:To study the optimal procedure of extracting vinblastine,catharanthine and vindoline from fresh Catharanthus roseus(L.) G.Don(bright-eyes,rose periwinkle,Madagascar periwinkle).Methods:The extraction method was optimized with orthogonal experimental design and RP-HPLC method was used to determine the contents of vinblastine,catharanthine and vindoline from fresh C.roseus.Results:The orthogonal experimental result showed that the optimal condition was as follows: ratio of fresh C.roseus(g) and solvent(mL) was 1∶ 10,extracting time of 14 hours and pH value was 0.5.Conclusion:It can increase the extraction yield of effective components from fresh C.roseus.The optimal method is simple and useful for the large-scale production.