Effects of 1-MCP on physiological and chemical characteristics of Agaricus Bisporus were studied through measuring the changes of peroxidase activity(POD), superoxide dismutase activity(SOD), catalase activity(CAT), malondialdehyde (MDA), soluble sugar and protein content after 1-MCP treatments.The results showed that:compared with the controls, the POD、 SOD、 CAT, soluble sugar and protein were respectively increased by 1950.00%,19.47%,389.42%,24.87% and 3.46% under 10μg/L concentrations of 1-MCP treatment, while MDA content was decreased by 51.08%.The POD,CAT,MDA, soluble sugar and protein were increased by 1150.00%,47.12%,20.29%,27.51% and 4.61% under 100μg/L concentrations of 1-MCP, while SOD was decreased by 2.07%.