Establishment of experimental animal model of osteoporosis with rat by ovariectomy

Objective To imitate bone structure of postmenopausal woman by establishing experimental animal model of osteoporosis with rats.Methods Twenty female SD rats were randomly divided into 2 groups: sham-operated group(n=10)and ovariectomized group(n=10) which were submitted to sham surgery or ovariectomy according to their designations.After 12 weeks densitometry of lumbar and tibiae was performed by dual-energy X-ray absorptiometry and tibiae was subjected to observation by SEM.Uterus was obtained and weighted.Results Densitometric analysis of the lumbar and tibiae confirmed a systemic impairment of animals,disclosing lower values(P0.05) of bone mineral density for OVX[(264.10±20.10)g/cm3,(163.20±11.15)g/cm3]in relation to the sham group[(302.50±25.92)g/cm3,(205.90±15.07)g/cm3].The observation under SEM supports results of bone density measurement.Weight of sham group was lower than OVX group,Uterus weight and coefficient of uterus were all higher than OVX group.Conclusion It was successful for establishment of experimental animal model of osteoporosis with rat by ovariectomy.