The polysaccharides were extracted from lentinus edodes,flammulina velutipes,volvaria volvacea,pleurotusostreatus,pleurotus eryngii and agrocybe cylindracea of six kinds of edible fungus using hot water extraction followed by ethanol precipitation.The content of polysaccharides ranged from 6.80±0.19 mg·g-1 to 31.87±0.27 mg·g-1 by phenol-sulfuric acid assay.The six kinds of polysaccharides exhibited typical absorption of polysaccharide characterized by IR spectroscopy,and showed the similar structural.