[Objective] To determine and compare the content of polysaccharides in tricholoma,plenrotus eryngii,white hypsizygus marmoreus and hypsizygus marmoreus.[Method]The polysaccharides obtained from four different edible fungus were extracted by water extraction and alcohol precipitation method.By using phenol-sulfuric acid method,glucose as the standard,the content of polysaccharides of the four different edible fungus were determined.[Result] The standard curve for glucose showed good linear,and its standard equation was y = 7.723 5x + 0.026 3,r = 0.997 8,and the content of polysaccharides in tricholoma,plenrotus eryngii,white hypsizygus marmoreus and hypsizygus marmoreus were 15.63%,52.38%,33.57%,31.79%.[Conclusion] The method is simple,accurate,and it has good stability.The content of polysaccharides were rich in the four different edible fungus,and plenrotus eryngii contained the highest content of polysaccharide among the four different edible fungus,and tricholoma contained the lowest content of polysaccharide,and the content of polysaccharides in white hypsizygus marmoreus and hypsizygus marmoreus were equal.