Effects of Qiliqiangxin capsule on H9C2 myocardial cell apoptosis induced by H_2O_2 in rats

Objective To investigate the effects of traditional Chinese medicine Qiliqiangxin capsule on H9C2 myocardial cell apoptosis induced by H_2O_2 in rats,and further to elucidate the molecular mechanisms.Methods The H9C2 myocardial cells were divided into the normal group,model group,Qiliqiangxin capsule treatment group. The model was established by injuring myocardial cell with 100μmol/L H_2O_2 for 6h.Each group were cultivated and measured the cell proliferation ratio by MTT method at different time points(12,24 and 48h).Cells were collected after 48h and subjected to RT-PCR and Western blot assays for the expression levels of Caspase-3,Bcl-2,Bax,Fas/FasL mRNA and protein.The percentage of apoptosis cells were detected by FCM.Results Compared with the model gronp,the rates of cell growth were significantly increased while the percentage of apoptosis cells were deceased in Qiliqiangxin capsule treatment group.The results of RT PCR and Western blot demonstrated that the expression of Caspase-3,Bax,Fas/FasL were decreased,and the expression of Bcl-2,Bcl-2/Bax were increased.Conclusion The traditional Chinese medicine Qiliqiangxin capsule could protect H9C2 from apoptosis through decreasing the expression of Caspase-3,Fas/FasL,and regulating the balance between Bcl-2 and Bax.