Identification of Cryptosporidium parvum 18S rRNA in food and water

Objective To isolate Cryptosporidium parvum oocysts from food and water and identify its 18S rRNA. Methods 78 food or water samples were enriched and Cryptosporidium parvum Oocysts was isolated. DNA was extracted and specific primers for the 18S rRNA of Cryptosporidium parvum was used in polymerase chain reaction(PCR). The amplified products were cloned and analyzed by PCR, digested with restriction enzyme and sequenced. Results 446bp PCR products were observed by agarose gel electrophoresis from the 78 samples. After cloning, the positive samples were identified by PCR and restriction enzyme digest. 99% of recombined clone DNA sequence were identical with 18S rRNA gene of Cryptosporidium parvum when NCBI-Blasting. Conclusion A method for genetic etection of Cryptosporidium parvum from food and water samples was established.