Objective To obtain the basic data of biological parameters of Beagle.Methods Routine methods and instrument are used to determine the biological parameters in Beagle using BECKMAN SYNCHRON CX5 PRO.ResultsThere were a few significant differences in Beagle between sexes(male and female Beagle)on parameters of CHOL、TG.Other indexes were no significant differences.ConclusionThere were no significant differences of some biological parameters in different sex and age.The all indexes were relatively stabilization.