干细胞
白血病
生物
细胞生物学
免疫组织化学
人口
抗体
分子生物学
癌症研究
免疫学
医学
环境卫生
作者
Kazuhito Naka,Yoshihiro Takihara
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 37-45
标识
DOI:10.1007/978-1-4939-4011-0_4
摘要
Traditionally, the intracellular localization and expression levels of specific proteins in CML Leukemia stem cells (LSCs) have been evaluated by fluorescence immunohistochemistry (FIHC). More recently, Duolink® in situ PLA technology has opened up a new and more quantitative way to evaluate signal transduction, posttranslational modification, and protein-protein interaction at the single-stem-cell level. This novel methodology, which employs two antibody-based probes, has already increased our understanding of the biology of the rare CML LSC population. In the future, the use of this approach may contribute to the development of novel therapeutics aimed at eradicating CML LSCs in CML patients.
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