色谱法
萃取(化学)
高效液相色谱法
化学
校准曲线
残留物(化学)
毒死蜱
固相萃取
吸光度
相关系数
农药残留
杀虫剂
检出限
数学
统计
生物
生物化学
农学
作者
Dave Harshit,Charmy Kothari,Nrupesh Patel
出处
期刊:Food Chemistry
[Elsevier]
日期:2017-09-01
卷期号:230: 448-453
被引量:149
标识
DOI:10.1016/j.foodchem.2017.03.083
摘要
The present work describes a novel HPLC and UV-spectrophotometric method for the simultaneous estimation of chlorpyrifos and prophenofos in vegetables and fruits. In UV method, the absorbance of chlorpyrifos and prophenofos was measured at wavelength 277nm and 289nm respectively and quantitation was carried out by first order derivative method. The calibration curve for chlorpyriphos and prophenofos was found to be linear in the range of 6-16μg/mL with correlation coefficient 0.9979 and 0.9993 respectively. The HPLC separation was achieved on Hibar C18 column (250×4.6mm, 5µ) using mobile phase consist of acetonitrile: water (90:10, v/v) at flow rate 1.0mL/min and the eluent was monitored at 219nm. Pesticides were extracted from the samples by solid phase extraction and liquid-liquid extraction before the analysis. Out of these two extraction methods, solid phase extraction was found more efficient in terms of % recovery as compared to liquid-liquid extraction. The developed method was applied for estimation of chlorpyrifos and prophenofos and it was found that the chlorpyrifos was 0.018mg/kg, which is above maximum residue limits (0.01mg/kg), which might be toxic to body. Prophenofos was not found in any collected samples of vegetables and fruits.
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