噬菌体疗法
鲍曼不动杆菌
生物
微生物学
毒力
噬菌体
长尾病毒科
抗生素耐药性
病毒学
多重耐药
溶解循环
抗生素
细菌
铜绿假单胞菌
基因
病毒
大肠杆菌
遗传学
作者
Yuyu Yuan,Lili Wang,Xiaoyü Li,Demeng Tan,Cong Cong,You-Hai Xu
出处
期刊:Virus Research
[Elsevier]
日期:2019-10-01
卷期号:272: 197734-197734
被引量:50
标识
DOI:10.1016/j.virusres.2019.197734
摘要
The control and treatment of multidrug resistant pathogens infections has become a grand challenge for clinicians worldwide. Virulent phage has long been considered as an effective bactericidal agent, which may be a potentially alternative to antibiotics. However, the rapid development of phage resistance seriously hinders the wide and continuous application of virulent phages. In this study, Acinetobacter baumannii phage vB_AbaS_D0 was isolated, characterized and used to control the phage resistance development in bacterial strains. Transmission electron microscopy analysis of vB_AbaS_D0 indicated it belonged to the Siphoviridae family with an icosahedral head. Its whole genome was 43, 051 bp in size, with a GC content of 45.48% and 55 putative open reading frames. The data showed that vB_AbaS_D0 was a virulent phage. Although vB_AbaS_D0 had a very weak bactericidal activity, a wide range of Acinetobacter baumannii strains were sensitive to it. The results suggested that the cocktail of vB_AbaS_D0 and another Acinetobacter baumannii phage vB_AbaP_D2 could improve the therapeutic efficacy in vivo and in vitro. The resistance mutation frequency of A. baumannii cells infected with D0 or phage cocktail was significantly lower than cells treated with D2 (P < 0.01). Phage therapy in the murine bacteremia model results showed that the percentage of phage resistant mutant occurrence in the phage D0 or cocktail treatment group was significantly lower than in phage D2 treatment group (P < 0.01).
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