生物
DNA条形码
系统发育树
有害生物分析
线粒体DNA
克莱德
单倍型
核苷酸多样性
遗传学
基因
进化生物学
植物
动物
基因型
作者
Abu Faiz Md Aslam,Sharmin Sultana,Sumita Rani Das,Abdul Jabber Howlader
出处
期刊:Bangladesh Journal of Zoology
[Bangladesh Journals Online]
日期:2019-12-12
卷期号:47 (2): 333-342
被引量:1
标识
DOI:10.3329/bjz.v47i2.44344
摘要
Tribolium confusum and Tribolium castaneum (Coleoptera: Tenebrionidae) are two very confusing pest species while identification is done on the basis of morphology only. Such pests are discovered in stored grain as immature stages, which further complicates the identification process. Accurate identification of these pests is urgently required for integrated pest management. In this research, DNA barcoding was used to identify these pests accurately at any life stage. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was analyzed. DNA barcode dataset of T. confusum (GeneBank Acc. no. MK120453.1) and T. castaneum (Acc. no. MK411585.1) were constructed. The nucleotide composition reveals that average AT contents (59.9%) were higher than the GC contents (38.6%). Phylogenetic analysis by maximum likelihood method showed that both the species were originated from a common major clade. About 17.13% nucleotide differences were noted between the CO1 sequences by multiple sequence alignment. The interspecies nucleotide genetic distance (0.200) was calculated using Kimura 2 parameter. Haplotype analysis showed high genetic diversity (112 mutaional steps) among them.
Bangladesh J. Zool. 47(2): 333-342, 2019
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