氨基甲酸酯
化学
有机磷
灭多威
色谱法
呋喃丹
乙酰甲胺磷
凝胶渗透色谱法
乙酰胆碱酯酶
聚合物
有机化学
酶
杀虫剂
生物
农学
作者
Dılek Nartop,Elvan Hasanoğlu Özkan,Nurdan Kurnaz Yetim,Nurşen Sarı
标识
DOI:10.1080/03601234.2020.1797425
摘要
In this study, new polymers containing amides (TrisPS-Ntaa, and TrisPS-Ntaa-Fc) were synthesized by condensation reaction for qualitative identification of insecticides. The synthesized polymers, including amides were investigated by infrared spectroscopy (IR), scanning electron microscopy-energy dispersion X- ray spectrometry (SEM-EDX), and gel permeation chromatography (GPC). Then, acetylcholinesterase enzyme (AChE) was covalently immobilized on these polymers to improve properties (including activity, reusability, and storage stability). Accordingly, organophosphate (malathion, acephate, chlorpyrifos methyl) and carbamate (carbofuran, methiocarb, methomyl), which are used to prevent harmful organisms in some agricultural products were enzymatically determined based on their inhibitory activity on AChE.
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