医学
口腔微生物群
牙龈炎
牙周炎
失调
发病机制
生物
内科学
微生物学
胃肠病学
疾病
微生物群
牙科
生物信息学
作者
Li Suhua,Zaker Rexidan,Chu Xueqian,Asihati Reyida,Li Choy Chong,Guo Xin,Jila Palidan,Sang Xiao-hong
出处
期刊:Current Bioinformatics
[Bentham Science]
日期:2021-07-31
标识
DOI:10.2174/1574893615999200728201624
摘要
Background: An improved comprehension of the oral microbiota function in the pathogenesis of disease will contribute to diagnosis and treatment for both hypertension and periodontal disease. In our study, a comparison of the salivary microbiome between hypertension and Non-hypertension cohorts was designed to reveal microbial signatures. <P> Methods: Patients were divided into four sub-groups: Gingivitis, Periodontitis (stage 2, 3 and 4). Then the hypertension and Non-hypertension cohorts were split into periodontal health and periodontitis subgroups. The salivary samples were processed for DNA extraction (n=246). The V3-V4 hypervariable regions of microbiome 16S rRNA genes were amplified. Finally, sequencing libraries was constructed and subjected them to bioinformatics and statistical analyses. <P> Results: The oral microbial diversity decreased in both hypertension and periodontal disease groups compared to the healthy. At the genus level, the diversity showed 100 different operational taxonomic units (OTUs) for differential abundance testing. The first trend showed OTUs decreased in relative abundance with increasing periodontal disease, and as well as hypertension groups and non-hypertensive. For this trend OTUs comprised a mix of primarily anaerobic commensals and potential acute diarrhea pathogens. The second trend was that the diversity of genera was decreased in hypertension relative to non-hypertension, including other anaerobic bacteria related with periodontal disease. <P> Conclusions: Microbiota diversity decreased in both hypertension and different stages of periodontal disease groups, however, Neisseria and Solobacterium genera increased in co-existing hypertension and periodontal disease. Obviously, these findings indicate that the abundance of genera continues to change due to additional stresses caused by co-existing conditions
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