Electrochemical Sensing of Ascorbate as an Index of Neuroprotection from Seizure Activity by Physical Exercise in Freely Moving Rats

Physical exercise (PE) has been drawing increasing attention to prevent and alleviate neural damage of brain diseases; however, in vivo sensing of the neuroprotection ability of PE remains a challenge. Here, we find that ascorbate can be used as a small molecular index for neuroprotective function of PE and the neuroprotection ability of PE can thus be in vivo monitored with an online electrochemical system (OECS) in freely moving animals. With the OECS as the sensing system, we find that the concentration of ascorbate in the microdialysate from the striatum increases greatly in kainic acid (KA)-induced seizure rats and reaches twice the basal level (i.e., 214.4 ± 32.7%, p < 0.001, n = 4) at a time point 90 min after KA microinjection. Such an increase of ascorbate is obviously attenuated (i.e., 153.6 ± 23.9% of the basal level, p < 0.05, n = 3) after PE, showing the neuroprotective activity of PE. This finding is believed to be significant in providing chemical insight into the neuroprotection ability of PE.