紧身衣
硫化氢
荧光
化学
光化学
滴定法
硫化物
选择性
组合化学
无机化学
硫黄
生物化学
有机化学
催化作用
量子力学
物理
作者
Huan Xiang,Tianqing Ye,Yanbo Li,Yaping Lin,Dan Liŭ,Hongwei Zhou,Jianbo Wang,Lei Li
出处
期刊:Molecules
[MDPI AG]
日期:2022-11-03
卷期号:27 (21): 7499-7499
被引量:4
标识
DOI:10.3390/molecules27217499
摘要
Hydrogen sulfide (H2S) as small molecular signal messenger plays key functions in numerous biological processes. The imaging detection of intracellular hydrogen sulfide is of great significance. In this work, a ratiometric fluorescent probe BH based on an asymmetric BODIPY dye for detection of H2S was designed and synthesized. After the interaction with hydrogen sulfide, probe display colorimetric and ratiometric fluorescence response, with its maximum emission fluorescence wavelength red-shifted from 542 nm to 594 nm, which is attributed to the sequential nucleophilic reaction of H2S leading to enhanced molecular conjugation after ring formation of the BODIPY skeleton. A special response mechanism has been fully investigated by NMR titration and MS, so that the probe has excellent detection selectivity. Furthermore, probe BH has low cytotoxicity and fluorescence imaging experiments indicate that it can be used to monitor hydrogen sulfide in living cells.
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