银纳米粒子
检出限
化学
硼酸
果糖
表面增强拉曼光谱
色谱法
苯硼酸
胶体金
尿
选择性
基质(水族馆)
纳米颗粒
核化学
拉曼光谱
组合化学
纳米技术
拉曼散射
生物化学
有机化学
材料科学
催化作用
地质学
物理
光学
海洋学
作者
Jialu Shen,Guoqing Chen,Zichen Yang,Yamin Wu,Chaoqun Ma,Lei Li,Taiqun Yang,Jiao Gu,Hui Gao,Chun Zhu
标识
DOI:10.1016/j.saa.2022.122179
摘要
The accurate detection of fructose in human urine can help prevent and screen for diseases such as fructokinase deficiency and hereditary fructose intolerance. Surface-enhanced Raman spectroscopy (SERS) is an analytical technique with selectivity and high sensitivity, which has been widely applied to the detection of targets with complex backgrounds. In this work, 4-mercaptophenylboronic acid (4-MPBA) was modified on the surface of silver nanoparticles (AgNPs) under mild conditions to obtain a boronic acid-functionalized SERS substrate for the detection of fructose in artificial urine. The detection mechanism was based on the deboronization reaction of 4-MPBA on the surface of AgNPs, which was induced by fructose, and the Raman signal of the generated thiophenol (TP) molecules was significantly enhanced by the silver nanoparticles, with a linear increase in SERS peak intensity at 1570 cm-1. We achieved the detection limits of 0.084 µmol/L in water and 0.535 µmol/L in urine by this method. The relative standard deviation (RSD) in the recovery experiments of urine ranged from 1.01 % to 2.22 %, and the whole detection time was less than 10 min, which indicated that this method is highly reliable for fructose detection and has a good prospect in bioassay and clinical medicine.
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