氧化应激
血脑屏障
外渗
脑损伤
炎症
医学
免疫印迹
内分泌学
埃文斯蓝
标记法
内科学
免疫组织化学
中枢神经系统
免疫学
生物
生物化学
基因
作者
Xuanpei Xu,Ruixi Zhou,Junjie Ying,Xiaoxue Li,Ruifeng Lu,Yi Qu,Dezhi Mu
出处
期刊:Peptides
[Elsevier]
日期:2023-03-01
卷期号:161: 170945-170945
被引量:4
标识
DOI:10.1016/j.peptides.2023.170945
摘要
Hypoxic-ischemic encephalopathy (HIE) is associated with excessive inflammation, blood-brain barrier dysfunction, and oxidative stress. Irisin can reduce inflammation and ameliorate oxidative stress; however, its effects on hypoxic-ischemic brain damage in newborns are unknown. Newborn Sprague-Dawley rats were subjected to hypoxic-ischemic injury and irisin treatment. TUNEL staining assays, the albumin-Evans blue dye extravasation method, an antioxidants detection kit, quantitative reverse-transcriptase PCR, enzyme linked immunosorbent assay, Western blot analysis, immunohistochemistry, and electron microscopy were used to investigate the possible mechanisms underlying the prevention of HIE by irisin. We discovered that rats affected by HIE and administered irisin had lower levels of IL-6 (but not TNF-α or IL-1β) less oxidative stress, and enhanced blood-brain barrier integrity. Irisin can effectively attenuate brain damage by reducing oxidative stress and protecting the blood-brain barrier.
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