铜绿假单胞菌
生物污染
生物膜
循环伏安法
介电谱
粘附
化学
玻璃碳
扫描电化学显微镜
原位
化学工程
电化学
腐蚀
材料科学
细菌
生物
有机化学
生物化学
电极
遗传学
物理化学
膜
工程类
作者
J. Pellé,Matthieu Longo,N. Le Poul,Claire Hellio,Stéphane Rioual,Benoît Lescop
标识
DOI:10.1016/j.bioelechem.2022.108344
摘要
Understanding and sensing microbial biofilm formation onto surfaces remains highly challenging for preventing corrosion and biofouling processes. For that purpose, we have thoroughly investigated biofilm formation onto glassy carbon electrode surfaces by using electrochemical technics. Pseudomonas aeruginosa was studied because of its remarkable ability to form biofilms in many environments. The modification of the electrode-solution interface during biofilm growth was monitored by in-situ measurement of the open-circuit potential and correlated with results obtained by electrochemical impedance spectroscopy, cyclic voltammetry, scanning electron microscopy and bioassays. The sensing of the biofilm formation hence suggests a multi-steps mechanism, which may include pre-formation of an insulating layer onto the surface prior to the bacteria adhesion and biofilm formation.
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