京尼平
伤口愈合
壳聚糖
自愈水凝胶
皮肤修复
化学
血管生成
生物物理学
材料科学
生物医学工程
高分子化学
生物化学
癌症研究
外科
医学
生物
作者
Rongtai Zuo,Jingjing Shi,Shu‐Heng Jiang,Min Chu,Qiyang Wang,Lingchi Kong,Qinglin Kang,Ya‐Jun Guo,Junjie Guan
标识
DOI:10.1016/j.ijbiomac.2022.12.022
摘要
Diabetic skin disorders are lingering and refractory clinical diseases. In this study, a genipin-crosslinked porous chitosan fiber (CSF) hydrogel was fabricated to achieve rapid wound healing. By embedding clemastine fumarate (CF) in the CSF hydrogel pores, we synthesised a CSF/CF hydrogel for the treatment of diabetic wounds. The microstructure, chemical elements, spectral variation, mechanical properties, swelling ratios, degradability, and toxicity of the CSF/CF hydrogels were studied. Compared with the typical CS power hydrogel, the porous CSF hydrogel crosslinked with genipin possesses a stable structure and improved physicochemical properties. Moreover, CF was slowly released from the CSF hydrogel. Molecular simulation also showed that CF was evenly embedded inside the cavity formed by the novel CSF hydrogel. The results suggested that CF can resist damage from high glucose levels and promote proliferation, tube formation, and migration of endothelial cells (ECs) and fibroblasts. The CSF/CF hydrogel promoted wound healing in a rat model. Mechanistically, the beneficial effect of CF on wound healing may be related to activation of the MEK/ERK and PI3K/Akt signalling pathways. In conclusion, genipin-crosslinked CSF/CF hydrogel can accelerate wound healing and may be an effective therapeutic method for treating diabetic skin lesions.
科研通智能强力驱动
Strongly Powered by AbleSci AI