The altermagnet candidate Mn5Si3 has attracted wide attention in the context of nonrelativistic spin physics, due to its composition of light elements. In this study, we demonstrate a hallmark of altermagnetism in Mn5Si3 thin films, namely the three options, or variants, for the checkerboard distribution of the magnetic Mn atoms. The magnetic symmetries were altered by field-rotation of the Néel vector along relevant crystal directions, resulting in anomalous Hall effect anisotropy. The experimental results in nanoscale devices were corroborated by a theoretical model involving atomic site dependent anisotropy and bulk Dzyaloshinskii-Moriya interaction for a single variant. These findings elevate Mn5Si3 from a candidate to a proven altermagnet. locked icon locked icon locked icon locked icon locked icon locked icon locked icon locked icon Physics Subject Headings (PhySH)Anisotropic magnetoresistanceAltermagnets