适体
检出限
溶解
细胞色素c
荧光
化学
细胞凋亡
污渍
猝灭(荧光)
分子生物学
生物物理学
色谱法
生物化学
生物
物理
量子力学
基因
作者
Hamed Salmani-Zarchi,Yasaman‐Sadat Borghei,Maryam Nikkhah
标识
DOI:10.1016/j.saa.2023.122933
摘要
To detect cytochrome c (Cyt c) as an important biomarker of apoptosis inside the cells, a simple, label-free, fluorometric detection method has been presented. For this purpose, an aptamer/gold nanocluster probe (Aptamer@AuNCs) was produced which could specifically bind to Cyt c leading to fluorescence quenching of AuNCs. The developed aptasensor showed two linear ranges of 1–80 μM and 100–1000 μM and a detection limit of 0.77 μM and 297.5 μM, respectively. This platform was successfully used to assay Cyt c release inside the apoptotic cells and their cell lysate. Aptamer@AuNC due to its enzyme-like properties could replace antibodies in Cyt c detection by conventional blotting techniques.
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