一致性
肺癌
微卫星不稳定性
医学
活检
DNA测序
前瞻性队列研究
肺
病理
深度测序
内科学
生物
肿瘤科
微卫星
等位基因
基因
遗传学
基因组
作者
Xiaosong Ben,Dan Tian,Weitao Zhuang,Rixin Chen,Sichao Wang,Zihao Zhou,Daizhan Cheng,Ruiqing Shi,Songlin Liu,Dongkun Zhang,Jiming Tang,Lei Xie,Haiyu Zhou,Zhou Zhang,Min Li,Xuanye Zhang,Guibin Qiao
标识
DOI:10.1186/s13000-022-01255-y
摘要
Successful practice of precision medicine in advanced lung cancers relies on therapeutic regimens tailored to individual molecular characteristics. The aim of this study was to investigate the accuracy of small specimens for molecular profiling using next-generation sequencing (NGS).Genetic alternations, tumor mutational burden (TMB), status of microsatellite instability (MSI), and expression of programmed death ligand 1 (PD-L1) were compared side-by-side between the concurrently obtained core needle biopsy (CNB) and resection specimens in 17 patients with resectable non-small cell lung cancers.DNA yield and library complexity were significantly lower in CNB specimens (both p < 0.01), whereas the insert size, sequencing depth, and Q30 ratio were similar between the matched specimens (all p > 0.05). The total numbers of genetic alternations detected in resection and CNB specimens were 186 and 211, respectively, with 156 alternations in common, yielding a specific concordance rate of 83.9%. The prevalence of mutations in 8 major driver genes was 100% identical between surgical and CNB specimens, though the allele frequency was lower in CNB specimens, with a median underestimation of 57%. Results of TMB were similar (p = 0.547) and MSI status was 100% matched in all paired specimens.Pulmonary CNB specimens were suitable for NGS given the satisfactory accuracy when compared to corresponding surgical specimens. NGS results yielding from CNB specimens should be deemed reliable to provide instructive information for the treatment of advanced lung cancers.
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