纳米探针
检出限
肌钙蛋白I
荧光
生物分子
免疫分析
纳米颗粒
光子上转换
费斯特共振能量转移
化学
材料科学
链霉亲和素
胶体金
纳米技术
色谱法
兴奋剂
生物素
光电子学
生物化学
抗体
心理学
物理
量子力学
精神科
心肌梗塞
免疫学
生物
作者
Merin K. Abraham,S. Madanan Anju,Susan Varghese,Ali Ibrahim Shkhair,Geneva Indongo,Greeshma Rajeevan,N. S. Vijila,Sony George
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2024-01-01
卷期号:149 (1): 231-243
被引量:1
摘要
Cardiac troponin I (cTnI) is a significant biomarker for acute heart attack. Hence, fast, economical, easy and real time monitoring of cardiac troponin I (cTnI) is of great importance in diagnosis and prognosis of heart failure in the healthcare domain. In this work, an immunoassay based on NaYF4:Yb/Ho based photon-upconversion nanoparticle (UCNP) with narrow emission peaks at 540 nm and 655 nm respectively, is synthesized. Then, it is encapsulated with amino functionalized silica using 3-aminopropyltriethoxysilane (APTES) to form APTES@SiO2-NaYF4:Yb/Ho UCNPs. When AuNPs is added to this system, the fluorescence is quenched by the electrostatic interaction with APTES@SiO2-NaYF4:Yb/Ho UCNPs, thereby exhibiting a FRET-based biosensor. When the cTnI antigen is introduced into the developed probe, an antibody-antigen complex is formed on the surface of the UCNPs resulting in fluorescence recovery. The developed sensor shows a linear response towards cTnI in the range from 0.1693 ng mL-1 to 1.9 ng mL-1 with a low limit of detection (LOD) of 5.5 × 10-2 ng mL-1. The probe exhibits adequate selectivity and sensitivity when compared with coexisting cardiac biomarkers, biomolecules and in real human serum samples.
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