Physical stability, microstructure and antimicrobial properties of konjac glucomannan coatings enriched with Litsea cubeba essential oil nanoemulsion and its effect on citruses preservation

This study purposed to develop konjac glucomannan (KGM) based antimicrobial coatings containing Litsea cubeba essential oil nanoemulsion (LNE) for citruses preservation. Physical stability, rheological, structural and antimicrobial properties of the coating solutions were investigated, along with the release characteristics of Litsea cubeba essential oil (LCO). Results showed that the coating solutions displayed shear thinning behavior. The oil droplets were distributed homogeneously in KGM phase with good stability. The coating structure became loose with increasing LNE content due to LNE interfering with molecular interactions and entanglement of KGM. The coating solutions showed stronger antibacterial activity against Escherichia coli than against Staphylococcus aureus and were effective in inhibiting the growth of Penicillium italicum on citrus surfaces. KGM-LNE 10 negatively affected citruses due to phytotoxicity caused by high levels of LCO. LCO was released slowly and continuously from the coatings, and its release was faster in deionized water than in an ethanol-water solution. KGM-LNE 2.5 coated citruses had the least weight loss, the greatest hardness, and kept the minimum changes in total soluble solids, total acid and vitamin C content, implying that KGM-LNE 2.5 best maintained the quality of citruses. The findings suggest that KGM-based coatings containing LNE have high potential for citruses preservation.