化学
三乙胺
色谱法
检出限
校准曲线
组胺
异硫氰酸苯酯
中心组合设计
析因实验
分式析因设计
高效液相色谱法
异硫氰酸盐
响应面法
有机化学
数学
医学
统计
内科学
作者
Maede Ameri,Seyed Mosayeb Daryanavard
出处
期刊:Journal of Chromatographic Science
[Oxford University Press]
日期:2023-08-02
标识
DOI:10.1093/chromsci/bmad060
摘要
Abstract Histamine as an important biogenic amino acid was measured in tuna fish samples by ultra-performance liquid chromatography using a phenyl isothiocyanate derivative. Minitab software was used to design the experiment and investigate the effective factors during the process, which includes screening and optimization steps. A partial factorial design was used in the screening stage and a central composite design was used in the optimization. Effective parameters in histamine derivatized were examined in the screening step including triethylamine volume, phenyl isothiocyanate volume, reaction temperature, reaction time and mobile phase pH. Then, in the optimization, effective parameters were identified and finally, the calibration curve was drawn from a concentration of 0.5–10.0 μg.mL−1 for histamine derivatized and a correlation coefficient of 0.994 was obtained for histamine derivatized. The method detection limit was 0.36 μg.mL−1 and the limit of quantification (LOQ) was 1.19 μg.mL−1. The relative standard deviation of the method was obtained for concentrations of 1.0–100.0 μg.mL−1 in the range between 1.06 and 2.21%. The recovery method was obtained from 90.8 to 103.1% for measuring histamine derivatized in real fish samples.
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