基因组编辑
清脆的
生物
基因组
计算生物学
遗传学
基因
作者
Ping Lv,Fei Su,Fangyuan Chen,Chunxue Yan,Dandan Xia,Hongyu Sun,Shanshan Li,Zhiqiang Duan,Changle Ma,Hui Zhang,Mugui Wang,Xiaomu Niu,Jian‐Kang Zhu,Jinshan Zhang
摘要
The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.
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