Optimization and purification of laccase activity from Mammaliicoccus sciuri isolated from the soils of Similipal, Odisha, India: a kinetics study of crystal violet dye decolorization

Four laccase-producing bacteria were found in soil samples from the Similipal Biosphere Reserve in Odisha, according to the current study. The isolates (SLCB1 to SLCB4) were evaluated for their laccase-producing ability in LB broth supplemented with guaiacol. The ABTS assay was performed to assess the laccase activity. The bacterium Mammaliicoccus sciuri shows the highest laccase activity i.e., 0.5125 U/L at the optimized conditions of pH 5.5, temperature 32.5 °C, ABTS concentration of 0.75 μl with an incubation time of 9 d. Laccase activity of M. sciuri grown in Sawdust was significantly increased in comparison to that in other agro wastes. The partially purified laccase enzyme after ammonium sulfate precipitation and dialysis showed a molecular weight of ∼58.5 kDa as determined by SDS-PAGE. A decolorization efficiency of 66.67% was recorded for the dye crystal violet after 1 h treatment with dialyzed laccase enzyme compared with phenol red, brilliant blue, and methylene blue.