A spatial-potential-resolved electrochemiluminescence biosensor for simultaneous detection of BRCA1 and BRCA2 based on a novel self-luminescent metal–organic framework

Conventional potential-resolved electrochemiluminescence (ECL) biosensors often require the participation of multiple co-reactants, which greatly limits their practical application. To solve the problem, a new kind of self-luminescent copper-based metal–organic framework (Cu-MOF) was synthesized for the first time, Cu-MOF can produce superior positive potential ECL without co-reactants. A novel spatial-potential-resolved electrochemiluminescence biosensor based on Cu-MOF and CdTe@CdS quantum dots (QDs) was constructed for simultaneous detection of BRCA1 and BRCA2. To achieve positive and negative potential luminescence at the same time, Cu-MOF and CdTe@CdS QDs were integrated into different locations of ITO electrode to achieve spatial-potential-resolved ECL only using one co-reactant, which had not been previously reported to my knowledge. Cu-MOF was placed in channel a as the signal luminophor, and Au@Ag NPs were used to quench the positive potential ECL of Cu-MOF by resonance energy transfer (RET) for detection of target BRCA1. Similarly, CdTe@CdS QDs as negative potential ECL material were introduced into channel b for detection of BRCA2. This work not only developed a novel self-luminescent ECL material, but also proposed an ingenious sensing strategy for simultaneous detection of multi-target, which has a wide application prospect in the field of biochemical analysis.