核孔蛋白
核孔
拟南芥
生物
拟南芥
细胞生物学
核板
核蛋白
核定位序列
核运输
细胞核
核心
遗传学
基因
突变体
转录因子
作者
Sarah Mermet,Maxime Voisin,Joris Mordier,Tristan Dubos,Sylvie Tutois,Pierre Tufféry,Célia Baroux,Kentaro Tamura,Aline V. Probst,Christophe Tatout,Christophe Tatout
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2023-09-21
卷期号:35 (12): 4284-4303
被引量:1
标识
DOI:10.1093/plcell/koad236
摘要
The nucleoskeleton forms a filamentous meshwork under the nuclear envelope and contributes to the regulation of nuclear shape and gene expression. To understand how the Arabidopsis (Arabidopsis thaliana) nucleoskeleton physically connects to the nuclear periphery in plants, we investigated the Arabidopsis nucleoskeleton protein KAKU4 and sought for functional regions responsible for its localization at the nuclear periphery. We identified 3 conserved peptide motifs within the N-terminal region of KAKU4, which are required for intermolecular interactions of KAKU4 with itself, interaction with the nucleoskeleton protein CROWDED NUCLEI (CRWN), localization at the nuclear periphery, and nuclear elongation in differentiated tissues. Unexpectedly, we find these motifs to be present also in NUP82 and NUP136, 2 plant-specific nucleoporins from the nuclear pore basket. We further show that NUP82, NUP136, and KAKU4 have a common evolutionary history predating nonvascular land plants with KAKU4 mainly localizing outside the nuclear pore suggesting its divergence from an ancient nucleoporin into a new nucleoskeleton component. Finally, we demonstrate that both NUP82 and NUP136, through their shared N-terminal motifs, interact with CRWN and KAKU4 proteins revealing the existence of a physical continuum between the nuclear pore and the nucleoskeleton in plants.
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